The analgesic effect of electroacupuncture in complete freund's adjuvant rats—a meta analysis☆

电针对完全弗氏佐剂实验鼠镇痛作用的整合分析

Kuo ZHANG（张阔）a, Zhi-fang XU（徐枝芳）b,c, Kai LI（李凯）d, Xue ZHAO（赵雪）b*, Bo CHEN（陈波）c*, Yi GUO（郭义）b,e, Shen-jun WANG （王慎军）b,c

a Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, 300072, China（天津大学医学工程与转化医学研究院，天津300072，中国）

b Experimental Acupuncture-Moxibustion Research Center, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China （天津中医药大学实验针灸学研究中心，天津301617，中国）

c Acu-moxibustion and Tuina Department, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China （天津中医药大学针灸推拿学院，天津301617，中国）

d Department of Gynaecology, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300381, China（天津中医药大学第一附属医院妇科，天津300381，中国）

e College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese

Medicine, Tianjin, 301617,China（天津中医药大学中医学院，天津301617，中国）

☆Supported by the National Natural Science Foundation of China: 81330088, 81303025.

∗ Corresponding author.

E-mail address: zhaoxue0216@126.com (Xue ZHAO)

F-tjutcmchenbo@163.com (Bo CHEN).

Abstract

Pain is a global problem and has been found sensitive to acupuncture. A large number of existing and newly published studies have demonstrated the analgesic effect of electroacupuncture (EA) in complete Freund’s adjuvant (CFA) rat model, necessitating a new and comprehensive review. To evaluate the curative effect of EA on thermal hyperalgesia in CFA rats, and to explore parameters that influence treatment effects, studies of EA treatment on thermal hyperalgesia of CFA rat model were identified from nine databases up to June 17, 2017. Outcome measure was heat pain threshold. All the data were analyzed using Stata 14.0/MP software. 26 studies identified included 801 rats. The quality score of the studies ranged from 3 to 6, with a mean of 4.08. The effect of EA group was improvement in outcome compared with CFA group. In subgroup analyses, frequency 2, 100, 2/100 or 20-100 Hz, acupoint Huántiào （环跳GB30）,Kūnlún（昆仑  BL60）+Xuánzhōng （悬钟 GB39）, Zúsānlĭ （足三里 ST36）+BL60 or ST36+Sānyīnjiāo（三阴交SP6）, retention time 20 or 30min, treatment time for post-CFA injection immediately, the same day as CFA injection, post-CFA injection 24 or 48h and interval 24 or 48h all can improve the effect on CFA rat heat pain threshold. These results show that there is positive effective of EA in CFA-induced pain, different parameters have different effects on EA treatment of CFA heat hyperalgesia, however, because of the number of eligible researches and the high risk of bias, the evidence supporting this conclusion is limited.

Key Words: electroacupuncture (EA), analgesia, complete Freund’s adjuvant, rats

[摘 要]疼痛是一个全球性问题，人们发现它对针灸很敏感。大量现有和新发表的研究证明了电针（EA）在完整的弗氏佐剂（CFA）实验鼠模型中的镇痛作用，因此有必要进行新的全面综述。为评估电针对弗氏佐剂实验鼠热痛觉过敏的疗效，并探讨影响治疗效果的参数，截至2017年6月17日，我们从9个数据库中确定了电针治疗对弗氏佐剂实验鼠热痛觉过敏的研究。结果测量为热痛阈值。所有数据均采用Stata 14.0/MP软件进行分析。26项研究包括801只实验鼠。研究的质量分数为3至6分，平均为4.08分。与弗氏佐剂组相比，电针组的效果是预后的改善。在亚组分析中，频率2、100、2 / 100或20-100 Hz，穴位环跳GB30，昆仑BL60）+Xuánzhōng（悬钟GB39，足三里ST36+ BL60或ST36 +三阴交SP6，保留时间20或30min，立即注射弗氏佐剂，弗氏佐剂注射当天，注射后24或48小时和间隔24或48小时的治疗时间均可以改善对弗氏佐剂实验鼠热痛的作用阈。这些结果表明，电针在弗氏佐剂诱发的疼痛中具有积极的疗效，不同的参数对电针治疗弗氏佐剂热痛觉过敏的效果不同，但由于符合条件的研究数量较多，且存在较高的偏倚风险，支持这一结论的证据有限。

[关键词]电针(EA)；镇痛；完全弗氏佐剂；实验鼠