王　佳１，高　琪１，徐东升１，张知云１，崔晶晶１，白万柱１*

（中国中医科学院针灸研究所, 北京100700）

摘要：激光扫描共聚焦显微镜（laser scanning confocal microscope，LSCM）是现代化的高精度显微镜，被广泛应用于各种研究领域，其中在针灸领域的应用已有二十余年。由于LSCM具有灵敏度和分辨率高以及可以三维重建和动态分析等优点，不仅可以从静态角度研究细胞结构，同时也可以从动态角度研究其特点。因此，在针灸形态学研究中，LSCM的使用得到不断普及，且目前已成为从组织学角度更好地揭示腧穴结构以及针灸效应的重要工具之一。本文将通过分析近年来LSCM在针灸研究方面的应用情况，揭示其在针灸形态学研究中的重要作用，同时，进一步展望LSCM在针灸研究中的应用前景。

关键词：针灸；激光扫描共聚焦显微镜；形态学；三维重建；可视化 

Recent Application of Laser Scanning Confocal Microscope for the Morphological Study of Acupuncture

 WANG Jia^１, GAO Qi^１, XU Dongsheng^１, ZHANG Zhiyun^１, CUI Jingjing^１, BAI Wanzhu^１*

(Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China)

Abstract: Objective: Laser scanning confocal microscope（LSCM）is a modern high-precision microscope. It has been widely used in various fields of research and has been applied in acupuncture for over 20 years. With the features of high sensitivity and resolution, three dimensional reconstruction, and dynamic signal analysis, LSCM can be applied for studying the cellular structure from the static and dynamic views, which make it popularly in the field of acupuncture research and to be one of the important tools for revealing the structure of acupuncture points and the effect of acupuncture from the perspective of histology. In the present paper, we summarized the previous works of the application of LSCM in the field of acupuncture research, and revealed its important role playing in this field. Meanwhile, we also look ahead its prospect in acupuncture field. 

Key words: acupuncture, laser scanning confocal microscope, morphology, cell, three dimensional reconstruction, visualization