Effect of moxibustion on the expression levels of proteins of neuron and neuropeptide in the intestinal tract of rats with Crohn's disease * 艾灸对克罗恩病大鼠肠道神经元及神经肽蛋白表达的影响 *

作者：孙梦晓 来源：本站原创 点击：1678次 更新：1523856484

　　<p style="text-align: justify;">QI Qin (祁&nbsp; 琴)¹,&nbsp; &nbsp;WU Lu-yi (吴璐一)¹,&nbsp; &nbsp;WU Huan-gan (吴焕淦)²,&nbsp; &nbsp;LIU Hui-rong (刘慧荣)², WANG Yuan-yuan (王圆圆)¹,&nbsp; &nbsp;LIU Ya-nan (刘雅楠)¹,&nbsp; &nbsp;WANG Cun (王&nbsp; 村)¹, HUANG Ru-de (黄儒德) ²,&nbsp; &nbsp;WANG Xiao-mei (王晓梅)²</p><p><br/></p><p style="text-align: justify;">1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 2. Shanghai Research Institute of Acupuncture and Meridian（1. 上海中医药大学，上海 201203，中国；2. 上海市针灸经络研究所）</p><p><br/></p><p><strong>ARTICLE INFO</strong></p><p>First author: QI Qin (1992-), female, postgraduate.</p><p>E-mail: 719928895@qq.com</p><p>Corresponding author: WANG Xiao-mei (1977-), female, post doctorate, master tutor.</p><p>Research field: clinical and basic research on acupuncture-moxibustion for gastrointestinal diseases.</p><p>E-mail: wxm123@vip.sina.com</p><p>* Supported by: Youth Scientific Research Project of Shanghai Municipal Commission of Health and Family Planning: 2013Q042; National Key Basic Research Development Program (973 Program): 2015CB554500; Support Plan of New Century Excellent Talents of Ministry of Education: NCET-13-0907; National Natural Science Foundation: 81574079; Shanghai Municipal Youth Science and Technology Venus Project: 16QA1403400.</p><p>Accepted on December 1, 2016.</p><p><br/></p><p><strong>ABSTRACT</strong></p><p style="text-align: justify;"><strong>Objective</strong>&nbsp; To explore the regulating effect of moxibustion on the enteric nervous system of rats with Crohn’s disease.</p><p style="text-align: justify;"><strong>Methods&nbsp;</strong> Ten SD rats were selected randomly from 40 rats as normal control (group A), and the other 30 rats were established into Crohn’s disease rat models by adopting clysis method with TNBS. On the basis of modeling successfully, the model rats were randomly divided into model group (group B), herbs-partitioned moxibustion group (group C) and mild moxibustion group (group D) with 8 rats in each group (4 rats were dead during modeling. After modeling, 2 rats were selected from group A, and 2 rats were selected from models for determination,at last, 8 rats were included in each group). In group C and group D, herbs-partitioned moxibustion or mild moxibustion was applied on “Tiānshū (天枢 ST 25)” bilaterally, and the rats in group A and group B were fixed as in treatment groups. HE stain was conducted, and morphological observation was performed on the colonic tissue of rats; the expression levels of proteins of S-100, SP, NPY and their receptors were observed by adopting immunohistochemical method. Results&nbsp; Compared with group A, the expression levels of proteins of S-100, SP and its receptor NK1R, NPY and its receptors NPY1R and NPY2R in the intestinal tract of rats in model groups increased obviously, and the differences were statistically significant. (PS-100&lt;0.01, PSP&lt;0.05, PNK1R&lt;0.01, PNPY&lt;0.05, PNPY1R&lt;0.05,&nbsp; PNPY2R&lt;0.01), after treatment with drug-paste interposed moxibustion and mild moxibustion, the levels reduced significantly (PS-100&lt;0.05, PSP&lt;0.05, PNK1R&lt;0.01, PNPY&lt;0.05, PNPY1R&lt;0.05, PNPY2R&lt;0.01). Conclusion&nbsp; Moxibustion treatment may regulate the expression levels of proteins of S-100, SP, NK1R, NPY, NPY1R and NPY2R through warm stimulation, alleviate inflammatory response of colonic tissue, and repair impaired colonic mucosa, thus achieving the goal of treating Crohn’s disease.</p><p><br/></p><p><strong>KEY WORDS</strong>: moxibustion; Crohn’s disease; S-100; substance P; NK1R; neuropeptide Y; NPY1R; NPY2R</p><p><br/></p><p><br/></p><p><strong>ABSTRACT IN CHINESE</strong></p><p><strong><br/></strong></p><p style="text-align: justify;">[摘 要] 目的：探讨艾灸对克罗恩病大鼠肠神经系统的调节作用。方法：将40只SD大鼠随机抽取10只作为正常对照组，其余30只应用TNBS灌肠法制备克罗恩病大鼠模型，在造模成功的基础上，将造模大鼠随机分为模型组、隔药灸组和温和灸组，每组8只（30只大鼠在造模过程中死亡4只，造模结束后，正常组和造模完成后的大鼠各随机抽取2只进行模型鉴定,最终每组8只）。隔药灸组和温和灸组分别采用隔药灸或温和灸天枢穴（双侧）治疗，正常组和模型组只作与治疗组相同的固定。采用HE染色，进行大鼠结肠组织形态学观察；采用免疫组化法观察大鼠结肠S-100、SP和NPY及其受体的蛋白表达水平。结果：与正常组比较，模型组大鼠结肠S-100、SP及其受体NK1R和NPY及其受体NPY1R、NPY2R的蛋白表达均明显升高且差异有统计学意义（S-100 P＜0.01，SP P＜0.05，NK1R P＜0.01，NPY P＜0.05，NPY1R P＜0.05，NPY2R P＜0.01），经隔药灸和温和灸治疗后，其均有显著的降低(S-100 P＜0.05，SP P＜0.05，NK1R P＜0.01，NPY P＜0.05，NPY1R P＜0.05，NPY2R P＜0.01）。结论：艾灸疗法可能通过温热刺激调节结肠S-100、SP、NK1R、NPY、NPY1R和NPY2R的蛋白表达，减轻结肠组织的炎症性反应，修复受损的结肠黏膜，从而达到治疗克罗恩病的作用。</p><p style="text-align: justify;">[关键词] 艾灸 克罗恩病 S-100 P物质 NK1R 神经肽Y NPY1R NPY2R</p><p><br/></p>